Discussion Category:  Miscellaneous

Bleeding hematoxylin

We use a LINISTAT Stainer for Mohs . I'm a trained histo tech but now I'm doing Mohs in a small lab. The reagent used in the Stainer are not the same as I used doing frozen sectioning in the past. The slide does not go into a fixative but into 95 then water . After hematoxylin,water and bluing , the tissue seem to bleed  blue down the slide. The tissue seems ok but it's not a nice clean slide. Any ideas of what I can change to clean up these slides

avatar placemark

Asked by

Maine Mohs Deb
Report this Post
avatar placemarkReply by
cuthrelle1930

You can try a warm water rinse instead of cold.  

Report this Post

Page 1 of 1