Discussion Category: Miscellaneous
Bleeding hematoxylin
We use a LINISTAT Stainer for Mohs . I'm a trained histo tech but now I'm doing Mohs in a small lab. The reagent used in the Stainer are not the same as I used doing frozen sectioning in the past. The slide does not go into a fixative but into 95 then water . After hematoxylin,water and bluing , the tissue seem to bleed blue down the slide. The tissue seems ok but it's not a nice clean slide. Any ideas of what I can change to clean up these slides