Home > Questions & Answers > Autozero doesnot zero the RI
9 months ago

Autozero doesnot zero the RI

I work on DMF, 80 degrees, try purging many times the RI and autozero is at -0.132V constant, so I cannot start running samples....

Any advice?



9 months agoReply from chmoris
avatar placemarkYes indeed, I have no columns at the moment and washing with 1 ml/min at a 1.3 Mbar. Washing reference size, pressure goes down to 0.7 Mbar

9 months agoReply from chromatology
avatar placemark Have you purged the reference side of the flow cell with your mobile phase first?

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