Discussion Category: Microplate Readers
Read Issues
During Fluo4 calcium assays the ligand will be dispensed, read for the first couple of columns. Despite dispensing ligand into the rest of the plate, the software stops detecting mid way through the plate and error message at the end of the run states 'more than one of your reads was saturated'
Reply byA&K
If you are saturating your samples then your instrument needs service and calbration. We are A&K Biosource and I can help. Search A&K Biosource and contact me.