Thermo Scientific - Varioskan Flash
Varioskan® Flash spectral scanning multimode reader
Varioskan® Flash includes fluorescence intensity, time-resolved fluorescence (TRF), photometric, and luminometric detection technologies. It provides optimal performance to speed up your assay development with unlimited wavelength selection, up to three onboard dispensers, unparalleled optical performance and the advanced SkanIt Software
Varioskan Flash is certified for DLReady™ by Promega* and LanthaScreen™ by Invitrogen Corp**.
Ordering Information:
5250030: Varioskan Flash with top reading
5250040: Varioskan Flash with top and Bottom reading
5250500: Varioskan LumiSens option, factory fitted (also enabling luminometric spectral scanning)
5250510: Dispenser option, with 1mL syringe, factory fitted
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Features of Varioskan Flash
Varioskan Flash spectral scanning multimode reader includes fluorescence intensity, time-resolved fluorescence (TRF), photometric, and optional luminometric detection technologies. It is designed for optimization and analysis of various assays, such as binding assays, ADMETox, molecular biology assays, enzyme kinetic studies, ion-channel and cell signaling assays.
- Unlimited wavelength selection for spectral analysis and measurement at any single wavelength—the optimal measurement wavelengths can be identified and easily selected for any assay at any time
- Extremely easy measurement setup with automated internal functions ensuring maximal sensitivity and full dynamic range for every assay
- Onboard dispensers for exact follow-up of kinetic reactions—essential for flash luminescence assays, Ca2+ flux studies and other rapid kinetic applications
- Increased assay throughput—reads up to 1536-well plates and can easily be integrated with automated systems
- High performance incubator for temperature critical assays
- Easy assay setup, flexible data handling and convenient report formatting with SkanIt Software
General Specifications
There are no General Specifications available.
Additional Specifications
Fluorescence Intensity/Time-Resolved Fluorescence
Sample format: 6 – 1536-well plates
Wavelength selection: Double excitation and double emission monochromators
Excitation wavelength range: 200 – 1000 nm
Emission wavelength range: 270 – 840 nm
Excitation/emission bandwidth: 5 nm and 12 nm/12 nm
Light source: Xenon flash lamp
Sensitivity/dynamic range: Fluorescence intensity, top reading: 6 decades, 384-well plate
Fluorescence intensity, bottom reading: 5.5 decades, 384-well plate
Time-resolved fluorescence, top reading: 6 decades, 384-well plate
Luminometry
Sample format: 6 – 1536-well plates, spectral scanning 6 – 384-well plates
Wavelength selection: All wavelengths, filters and double monochromators
Wavelength range: 360 – 670 nm, spectral scanning 270 – 840 nm
Sensitivity/dynamic range: 7 decades, flash ATP reaction, 384-well plate
Photometry
Sample format: 6 – 384-well plates
Wavelength selection: Double monochromators
Wavelength range: 200 – 1000 nm
Bandwidth: 5 nm
Light source: Xenon flash lamp
Linear measurement range: 0 – 4 Abs (96-well plate) at 450 nm, ± 2%
0 – 3 Abs (384-well plate) at 450 nm, ± 2%
Accuracy: ± 2% or 0.003 Abs, whichever is greater, at 200 – 399 nm (0 – 2 Abs)
± 1% or 0.003 Abs, whichever is greater, at 400 – 1000 nm (0 – 3 Abs)
Precision: SD < 0.001 Abs or CV < 0.5%, whichever is greater, at 450 nm (0 – 3 Abs)
Dispenser
Number of dispensers: Up to 3, automatic dispensing position control
Sample format: 6 – 384-well plates
Syringe size: 1 ml (standard), 5 ml (on request)
Dispensing volume 1 – 10 000 µl, with 1 µl increments (1 ml syringe)
Automatic safety control based on maximum well volume
Accuracy: < 0.2 µl or 2%, whichever is greater, 5 – 10 000 µl (1 ml syringe, 0.40 mm tip)
Precision: 5 – 19 µl < 5%, 20 – 10 000 µl < 2% (1 ml syringe, 0.40 mm tip)
Dispensing speed: 30 s for 96-well plate; 80 s for 384-well plate (5 µl/well, 1 ml syringe, 0.40 mm tip)
Incubator: From ambient + 4°C to 45°C at ambient 25°C
Shaker: Orbital with adjustable speed and diameter
General Features
Measurement modes: Endpoint, kinetic, spectral scanning with all detection technologies
Measurement speed: 96-well plate in 15 s, 384-well plate in 45 s, and 1536-well plate in 135 s (minimum kinetic interval time from A1 back to A1)
Spectral scanning speed: < 2 s/well, 400 – 500 nm, 1 flash, 2 nm steps
Series Modals
Thermo Scientific - FI/TRF Specifications
[+] View Model Specifications| Bandwidth | Excitation/emission: 5 nm and 12 nm/12nm |
| Depth | 22.8 in |
| Height | 19.7 in |
| Detection Mode | Multi-label |
| Width | 21.3 in |
| Shaking Pattern | Orbital with adjustable speed and diameter |
| Sample Formats | 6 – 1536-well plates |
| Lighting | Xenon flash lamp |
| Read Method | Endpoint, kinetic, spectral scanning with all detection technologies |
| Scanning Speed | 96-well plate in 15 s, 384-well plate in 45 s, and 1536-well plate in 135 s (minimum kinetic interval time from A1 back to A1). Spectral scanning speed: < 2 s/well, 400 – 500 nm, 1 flash, 2 nm steps |
| Wavelength Selection | Double excitation and double emission monochromators |
| Additional Model Specifications | Sensitivity/dynamic range: Fluorescence intensity, top reading: 6 decades, 384-well plate Fluorescence intensity, bottom reading: 5.5 decades, 384-well plate Time-resolved fluorescence, top reading: 6 decades, 384-well plate Dispenser Number of dispensers: Up to 3, automatic dispensing position control Syringe size: 1 ml (standard), 5 ml (on request) Dispensing volume 1 – 10 000 µl, with 1 µl increments (1 ml syringe) Automatic safety control based on maximum well volume Accuracy: < 0.2 µl or 2%, whichever is greater, 5 – 10 000 µl (1 ml syringe, 0.40 mm tip) Precision: 5 – 19 µl < 5%, 20 – 10 000 µl < 2% (1 ml syringe, 0.40 mm tip) Dispensing speed: 30 s for 96-well plate; 80 s for 384-well plate (5 µl/well, 1 ml syringe, 0.40 mm tip) |
Thermo Scientific - Luminometry Specifications
[+] View Model Specifications| Depth | 22.8 in |
| Height | 19.7 in |
| Detection Mode | Multi-label |
| Width | 21.3 in |
| Shaking Pattern | Orbital with adjustable speed and diameter |
| Sample Formats | 6 – 1536-well plates, spectral scanning 6 – 384-well plates |
| Read Method | Endpoint, kinetic, spectral scanning with all detection technologies |
| Scanning Speed | 96-well plate in 15 s, 384-well plate in 45 s, and 1536-well plate in 135 s (minimum kinetic interval time from A1 back to A1) |
| Wavelength Selection | All wavelengths, filters and double monochromators |
| Additional Model Specifications | Sensitivity/dynamic range: 7 decades, flash ATP reaction, 384-well plate Dispenser Up to 3, automatic dispensing position control Plate types: 6 – 384-well plates Syringe size: 1 ml (standard), 5 ml (on request) Dispensing volume: 1 – 10 000 µl, with 1 µl increments (1 ml syringe). Automatic safety control based on maximum well volume Accuracy: < 0.2 µl or 2%, whichever is greater, 5 – 10 000 µl (1 ml syringe, 0.40 mm tip) Precision: 5 – 19 µl < 5%, 20 – 10 000 µl < 2% (1 ml syringe, 0.40 mm tip) Dispensing speed: 30 s, 96-well plate, 80 s, 384-well plate (5 µl/well, 1 ml syringe, 0.40 mm tip) Spectral Scanning Speed: < 2 s/well, 400 – 500 nm, 1 flash, 2 nm steps |
Thermo Scientific - Photometry Specifications
[+] View Model Specifications| Bandwidth | 5 nm |
| Depth | 22.8 in |
| Height | 19.7 in |
| Detection Mode | Multi-label |
| Width | 21.3 in |
| Sample Formats | 6 – 384-well plates |
| Lighting | Xenon flash lamp |
| Wavelength Selection | Double monochromators |
| Additional Model Specifications | Linear measurement range: 0 – 4 Abs (96-well plate) at 450 nm, ± 2% 0 – 3 Abs (384-well plate) at 450 nm, ± 2% Accuracy: ± 2% or 0.003 Abs, whichever is greater, at 200 – 399 nm (0 – 2 Abs) ± 1% or 0.003 Abs, whichever is greater, at 400 – 1000 nm (0 – 3 Abs) Precision: SD < 0.001 Abs or CV < 0.5%, whichever is greater, at 450 nm (0 – 3 Abs) Dispenser Up to 3, automatic dispensing position control Plate types: 6 – 384-well plates Syringe size: 1 ml (standard), 5 ml (on request) Dispensing volume: 1 – 10 000 µl, with 1 µl increments (1 ml syringe). Automatic safety control based on maximum well volume Accuracy: < 0.2 µl or 2%, whichever is greater, 5 – 10 000 µl (1 ml syringe, 0.40 mm tip) Precision: 5 – 19 µl < 5%, 20 – 10 000 µl < 2% (1 ml syringe, 0.40 mm tip) Dispensing speed: 30 s, 96-well plate, 80 s, 384-well plate (5 µl/well, 1 ml syringe, 0.40 mm tip) Spectral Scanning Speed: < 2 s/well, 400 – 500 nm, 1 flash, 2 nm steps |