I have quite an annoying problem with a Beckman Optima TLX 120 000 ultracentrifuge concerning the SPD alert. I use the 100.2 rotor.
A month ago, I wanted to centrifuge for 1h at 15 000 rpm at 12 degres so I filled the rotor with tubes balanced up to mg and I got the SPD alert 6 times that day!! With the 20 minutes wait in between each. But then when I tested the empty rotor it was working...I don't understand why the tubes would be problematic since I balanced them very precisely. A collegue told me about the room temperature, that if it is too cold in the room it could be problematic, does anyone have any info on that???
I tested it again this morning, the empty 100.2 rotor works fine, then I filled it with tubes containing 1 ml H2O precisely balanced the first start it got stuck at 4500-4800 rpms without the SPD alert, I stopped it and restart and that time all fine...I can't seem to find the problem and the Beckman support guy neither so please help me!!
Thanks a lot! Reply to marieboulet RE: Beckman Coulter - Optima™ TLX