Applied Biosystems - StepOne™
The StepOne™ Real-Time PCR System is a 48-well, low-throughput Real-Time PCR instrument perfect for both first-time and experienced users.
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Features of StepOne™
- Advanced software and instrumentation for performing a wide array of genomic assays.
- Sensitive 3-color optical LED recording system.
- Intuitive and robust software perfect for first-time and advanced users.
- Simple and flexible instrument set-up and usage.
General Specifications
| Depth | 16.8 in |
| Temperature Uniformity | 0.5 °C |
| Height | 20.2 in |
| Real-Time PCR | 1 |
| Width | 9.7 in |
| Power Requirements | 120 VAC ± 10%; 50/60 Hz ± 1%; 8A |
| Peak Power | 950 W |
| Weight | 52 lbs |
| Temp. Differential Range | 4 to 100 °C |
| Temperature Accuracy | 0.25 °C |
Additional Specifications
Thermal Cycling System: Peltier-based system
Block Format: 48-well block
Compatible Consumables:
48-well plates with optical flat caps
48-well plates with optical adhesive cover
8-tube (0.1 mL) strips with optical flat caps
Individual (0.1 mL) tubes with optical flat caps
Supported Volumes: 10–30 ΜL
Sample Ramp Rate:
Fast mode: +/- 2.2 °C/sec
Standard mode: +/- 1.6°C/sec
Peak Block Ramp Rate: 4.6 °C/sec
Temperature Accuracy: +/-0.25°C (35°C to 95°C) of setpoint/display temperature, measured at 3 minutes after clock start.
Temperature Uniformity: +/-0.50°C, 30 seconds after clock start.
Melt Curve Resolution: As small as 0.1°C
Optical System: LED, emission filters, and photodiode
Calibrated Dyes at Installation: SYBR® Green I, FAM™, VIC®, JOE™, ROX™ dyes
Passive Reference Dyes: ROX™ or any calibrated dye. Option exists to select no passive reference.
Data Collection: Data collected in all 3 filters for all wells regardless of plate setup.
Plate setup may be altered after run completes.
Fast cycling: Less than 40 minutes
Quantitative PCR Run Time: Standard cycling: Less than 2 hrs
Fast cycling: Less than 40 minutes
Touchscreen: LCD/6.5 in. Full VGA (640x480)/32K colors
Software: System Software included at no additional cost.
Applications include Standard Curve, Relative Standard Curve, Comparative Ct (DDCt), Allelic Discrimination (Genotyping), Melt Curve, Presence/Absence (Plus/Minus). Unlimited number of software licenses provided with each instrument system. Primer Express Software 3.0 (to custom design primers and probes) included at no additional cost.