Thermo Scientific - UltiMate 3000 Basic Manual
Enjoy cost effective access to UHPLC separations in a simple, expandable system where modularity makes anything...
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Features of UltiMate 3000 Basic Manual
Recommended Use For laboratories that need an LC with entry level UHPLC capabilities, and are investing in their first systems. The quaternary pump is recommended for methods deploying either conventional 3-5µm, or sub-2µm particle UHPLC columns where a moderately fast gradient response is acceptable for the benefit of increased mobile phase flexibility (i.e., multiple solvents, extended column wash, ternary or quaternary gradients). A four-channel solvent degasser is integrated in the pump module to simplify operation.
Typical application areas are teaching laboratories within general academic research and quality control laboratories with low sample throughput requirements.
Ensuring Excellent Chromatography
Up to 10 mL/min flow rate supporting up to 62 MPa (9000 psi/620 bar) backpressure for column format and column particle size flexibility. * Manual or motorized injection valve with contact closure for automated data acquisition start (i.e., Chromeleon 6.x/7.x) * Obtain near-zero dead volume for optimal peak shapes even over extended periods using Thermo Scientific™ Dionex™ Viper™ Fingertight Fitting system. * Get precise and accurate flow rates for stable retention times, robust peak integration, and confident identification from the Thermo Scientific™ Dionex™ SmartFlow™ technology.
Chromeleon CDS Software Makes Operation Easy * Integrated service and validation monitoring with Thermo Scientific™ Dionex™ Chromeleon™ CDS, versions 6.x and 7.x * Automated system startup, standby and shutdown with Chromeleon CDS, versions 6.x and 7.x * Compatible with Chromeleon 7.x eWorkflow walk-up solution and advanced reporting features
Choose the Right Configuration for You * Four-solvent channel pump for versatile gradient methods, with integrated 4-channel degasser * (LPG-3400SD) or a single solvent channel pump for isocratic applications without degassing (ISO-3100SD) * Spacious solvent rack for six 1-liter solvent bottles or large mobile phase reservoirs (SR-3000) Optional motorized injection valve supporting backpressures up to 1000 bar (i.e., with UltiMate 3000 RS pumps) * Thermostatted column compartment with up to two field upgradable column switching valves (TCC-3000SD) * Diode array, multiple wavelength, variable wavelength, fluorescence, charged aerosol, refractive index or mass spectrometry detectors * Fraction collector (AFC-3000) * Chromeleon 7.2 CDS software package (standalone or networked, may control also your other chromatography equipment)
General Specifications
Detector | Variable Wavelength |
Maximum Pressure | 9000 psi |
Standard Flow Rate | 0.2 to 10 ml/min |
Additional Specifications
PUMPS
Recommended Pump: LPG-3400SD
Flow Range: (Settable Range) 0.2–10 mL/min (0.001–10 mL/min)
Flow Accuracy: ±0.1%
Flow Precision: <0.05% RSD or <0.01 min SD, whichever is greater
Pressure Range: 2–62 MPa (290–9000 psi)
No. of Eluent Lines: 4
Gradient Delay Volume: 690 µL by default (325–1790 µL with optional mixer kits), independent of operating pressure
Biocompatible Version: LPG-3400AB (titanium)
Solvent Degassing: Built-in, 4 channels
DETECTOR
Recommended Detectors: VWD-3100
Detector Type: Variable Wavelength
Number of Channels: 1
Wavelength Range: 190–900 nm
Max. Data Collection Rate: 100 Hz
Recommened Flow Cell: Analytical: 11 µL, 10 mm path
Noise: <±3.5 µAU at 254 nm
Drift: <0.1 µAU/h