Shimadzu - Fuel-Grade Ethanol Production
HPLC for the Ethanol Production Laboratory
HPLC is the most commonly used technique for this analysis, as it makes use of both the stationary phase and the solvent (mobile phase) to effect control over the separation. In the case of the ethanol production plant, the HPLC system is typically used to profile the carbohydrate, alcohol, and organic acid content of the fermentation broth.
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Features of Fuel-Grade Ethanol Production
A basic isocratic HPLC system is typically used for Ethanol analysis during the production process. The components normally include:
- The mobile phase, a dilute solution of sulfuric acid (0.002-0.005N)
- An isocratic pumping system
- An autosampler
- A suitable chromatographic column
- A refractive index detector
- A data acquisition system
Most systems also include a mobile phase degasser to help eliminate out-gassing from the mobile phase, a column heater and a temperature controlled sample compartment for sample integrity.
Calibration of the HPLC system by use of a standard solution of the components of interest allows the users to obtain results as weight percent for the analytes of the broth samples. This data can be used to evaluate the progress of the fermentation and what intervention may be necessary to maximize the production of ethanol.
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