Thermo Scientific - FI/TRF Specifications
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General Specifications
Bandwidth | Excitation/emission: 5 nm and 12 nm/12nm |
Depth | 22.8 in |
Height | 19.7 in |
Detection Mode | Multi-label |
Width | 21.3 in |
Shaking Pattern | Orbital with adjustable speed and diameter |
Sample Formats | 6 – 1536-well plates |
Lighting | Xenon flash lamp |
Read Method | Endpoint, kinetic, spectral scanning with all detection technologies |
Scanning Speed | 96-well plate in 15 s, 384-well plate in 45 s, and 1536-well plate in 135 s (minimum kinetic interval time from A1 back to A1). Spectral scanning speed: < 2 s/well, 400 – 500 nm, 1 flash, 2 nm steps |
Wavelength Selection | Double excitation and double emission monochromators |
Additional Specifications
Sensitivity/dynamic range: Fluorescence intensity, top reading: 6 decades, 384-well plate
Fluorescence intensity, bottom reading: 5.5 decades, 384-well plate
Time-resolved fluorescence, top reading: 6 decades, 384-well plate
Dispenser
Number of dispensers: Up to 3, automatic dispensing position control
Syringe size: 1 ml (standard), 5 ml (on request)
Dispensing volume 1 – 10 000 µl, with 1 µl increments (1 ml syringe)
Automatic safety control based on maximum well volume
Accuracy: < 0.2 µl or 2%, whichever is greater, 5 – 10 000 µl (1 ml syringe, 0.40 mm tip)
Precision: 5 – 19 µl < 5%, 20 – 10 000 µl < 2% (1 ml syringe, 0.40 mm tip)
Dispensing speed: 30 s for 96-well plate; 80 s for 384-well plate (5 µl/well, 1 ml syringe, 0.40 mm tip)