Routine Exact Mass Measurement      

• Identify proteins or determine the elemental compositions of small molecules using routine sub 3 ppm RMS mass measurements      
• Obtain exact mass data without spectral interference or suppression effects using the LockSpray™ ion source      
• Target on the correct molecular formulae with i-FIT™ Software, which considers both exact mass and isotope patterns when reporting elemental compositions      
• Gain confidence from a spectral resolution in excess of 12,000 FWHM, which enables analyte peaks to be separated from interfering ions that might cause significant errors in exact mass determination      

Versatile Operation      

• Easily switch between ESI, APcl, ESC® and APPI ionization options      
• Choose from LockSpray and nanoLockSpray™ sources for either normal flow or nanoflow chromatography      
• Obtain greater coverage for screening applications by rapidly switching between positive and negative ionization modes during LC/MS analyses      
• Keep up with the highly resolved and rapidly eluting peaks associated with ACQUITY UPLC® chromatography with a data acquisition rate of up to 20 spectra/sec      
• Perform non-target compound screening with the ChromaLynx™ Application Manager      
• Quickly identify drug metabolites and degradation products with the MetaboLynx™ Application Manager      
• Analyze complex datasets for metabonomic and other chemometric experiments using the MarkerLynx™ Application Manager      

The LCT Premier XE’s sensitivity, fast acquisition and exact mass capability make it ideal for a variety of applications.      

Investigation of Non-Covalently Bound Protein Complexes      

ESI spectrum of proteasome 20S, a 700 kDa protein complex composed of 28 subunits arranged in four heptameric, tightly stacked rings to form a cylindrical structure.

Sensitivity      

Analyze compounds at trace levels using the sensitivity afforded by dual-stage ion transfer optics      

Atmospheric Solids Analysis Prove      

The Atmospheric Solids Analysis Probe (ASAP) is a powerful tool for the rapid direct analysis of volatile and semi-volatile, solid and liquid samples using atmospheric pressure ionization.      

The ASAP technique utilizes the heated nitrogen desolvation gas to vaporize the sample and a corona discharge for sample ionization. This allows low polarity compounds not amenable to ESI, APcI, and APPI to be ionized with a high degree of sensitivity. Furthermore complex mixtures can be analyzed without the need for any sample preparation.      

The Atmospheric Solids Analysis Probe is readily fitted to a standard API source by replacing either the ESI or APcI probe assembly. A corona discharge pin also requires fitting. The close proximity of the sample, when loaded onto a glass capillary tube, to the point of ionization and the MS inlet, improves sensitivity whilst also ensuring safety due to the enclosed source housing.      

Instruments are operated in combined Electrospray/APcI mode (ESCi®). This provides for the acquisition of both analyte data in APcI mode and reference data in electrospray mode, enabling exact mass measurements to be produced.      

The probe is designed to have both fixed & removable sections to allow rapid sample introduction without removal of the complete assembly ensuring stable source conditions.      

Routine Exact Mass      

Compound X was analyzed via repeat LC/MS injections over a course of 10 days. The mean RMS mass measurement error for these data = 1.9 ppm.

Sensitivity      

Sensitivity achieved from 10 pg of reserpine injected on column.