The Highest Resolution Imaging of Any BioAFM — Any Time, Every Time 

Double helix DNA — The unique mechanical stability and performance of the BioScope Resolve AFM on an optical microscope enables researchers to routinely resolve the major and minor grooves along the backbone of an individual DNA strand. 

High-resolution cell imaging — With the BioScope Resolve and exclusive ScanAsyst®, high-resolution cell imaging has never been easier. This game-changing capability has allowed soft, flexible cell surface structures, such as microvilli, to be resolved for the first time on living cells by AFM. 

Complete Cell Mechanics Data – Accurate, Repeatable 

Ultimate force control — PeakForce QNM®,has become the preferred mode for nanomechanical mapping. With new algorithms, it delivers highest resolution property maps at pN forces on live cells. Together with “No Touch” tip calibration, PeakForce QNM delivers the fastest quantitative mechanical characterization of live cells, providing a complete force curve for every pixel at actuation rates from 125 Hz to 2 kHz. 

Widest range of ramp rates – At the core of quantitative nanomechanical maps are force-distance curves. Beyond characterizing elastic response, ramp rate dependent data provides insight into viscoelasticity or into molecular interaction times at the single molecule level. With our new FASTForce Volume covering rates from sub-Hz to 300 Hz, and PeakForce Tapping extending to 2 kHz, we provide the widest range of ramp rates for single points and imaging while maintaining pN force control. 

Covering the whole spectrum — Our new RampScript™ allows the user to build, control and record complex nanomechanical measurements for use in protein pulling, ligand-receptor interaction, cell relaxation and viscoelastic probing. Its low force trigger capability, and latency-free implementation build on the system’s core performance to provide the most accurate script execution and force control. Ramping scripts are created in an intuitive drag and drop process and provide ultimate flexibility including per segment optical synchronization. 

Investigating Cell Dynamics – Without Compromise 

Fast Tapping on living cells - Bioscope Resolve is the only BioAFM that enables fast scanning while simultaneously providing the large piezo scanner range necessary for capturing the dynamic behavior of living cells in real time (XYZ = 100µm x 100µm x 15µm). The Fast Tapping capabilities of Resolve are truly a step forward in conducting high-resolution AFM studies at timescales relevant to cellular processes. Together with the integration of advanced optical microscopy and environmental control, live cell studies have never been easier. 

Dynamics of cell migration – High-resolution Fast Tapping movies enable direct observation of membrane ruffling events and associated dynamic rearrangement of the actin cytoskeleton at the leading edge of a living endothelial cell. Images were obtained at 35 seconds/frame (left – 37µm scan size) and 12.5 seconds/frame (right - 27µm scan size). 

Combined Fluorescence and Biomechanics – Seamless, Simple 

Expanded measurement possibilities — The new MIROView GUI ensures expert data generation via fully synchronized AFM images, force maps, and single-force curves with optical images and data.  

Great optical integration — BioScope Resolve AFM works seamlessly with an optical microscope, thanks to communication protocols developed with researchers’ applications in mind. Pixel by pixel, line by line, and image by image, optical synchronization delivers flexibility never seen before on an AFM. 

The perfect environment — With a new acoustic hood, work station, and a complete suite of life science research accessories, such as a sample heater, perfusion cell, and a top-down camera, the BioScope Resolve provides all your bioAFM research needs.