Thermo Scientific - Fluoroskan Ascent FL
Combined microplate fluorometer and luminometer for a variety of applications
Fluoroskan Ascent FL is a compact and robust instrument with excellent optical performance for a variety of fluorometric and luminometric research applications offering versatile plate formats, fast reading speeds, up to three dispensers and top / bottom reading of plates.
5210450: Fluoroskan Ascent FL 100-240V, 50/60Hz
5210460: Fluoroskan Ascent FL 100 - 240V, 50/60Hz with one dispenser
5210462: Fluoroskan Ascent FL 100-240V, 50/60Hz with two dispensers
5210463: Fluoroskan Ascent FL 100-240V, 50/60Hz with three dispensers
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Features of Fluoroskan Ascent FL
Fast reading speed
- Reads a 96-well plate in just 15 seconds, which is essential for kinetic applications
- Great for a wide variety of applications, such as cytotoxicity, ion channel, FRET and BRET
- Combination of fluorometric GFP measurement and a lumometric luciferase measurement from the same well is fast due to the versatility of assay programming
Onboard dispensers for exact follow-up of kinetic reactions
- Equipped with up to three reagent dispensers for rapid kinetic assays, such as Ca2+ flux
- Supports simultaneous dispensing and reading, enabling monitoring of kinetic measurements from the very start of the reaction
- Very low dead volume and the backflush capability allow conservation of expensive reagent
High sensitivity for both top and bottom reading
- Fiberless direct illumination optics for both top and bottom reading
- High sensitivity, wide dynamic range and low cross talk ensure accurate and precise results for 1- to 384-well plates
Specially designed for automation
- Reads up to 384-well plates for increased assay throughput
- Easily integrated with automated systems
Includes: PC Software and filter pairs: Excitation: 355nm/Emission: 460nm, Excitation: 485nm/ Emission: 538nm Warranty and Service Offering: 1 year Recommended for: Ca2+ flux assays; Cell proliferation; Cytotoxicity; Cell adhesion; DNA quantitation; Reporter gene assays; Hybridization assays; Quantitation of PCR products; FRET assays; BRET assays; Molecular beacon assays; Immunoassays; Enzyme activity; Bacterial quantitation; Phagocytosis; Oligonucleotide assays
General Specifications
There are no General Specifications available.
Additional Specifications
Note: 3 optional dispensers add 7.7 lbs to the weight.
Fluorometry
Excitation wavelength range: 320-700 nm
Emission wavelength range: 360 - 670 nm
Excitation filters: Up to eight in the excitation filter wheel. 355 nm and 485 nm filters included as standard. Other filters available upon request.
Emission filters: Up to six filters in the emission filter wheel.
460 nm and 538 nm filters included as standard. Other filters available upon request.
Sensitivity: 2 fmol fluorescein/well in a black 96-well plate
Dynamic range: >6 decades
Luminometry
Spectral range: 270 - 670 nm
Sensitivity: 40 amol ATP/well using flash reaction, white 384-well plate
Dynamic range: > 9 decades over whole gain setting area
Dispensing
Number of dispensers: Up to 3
Dispensing volume: 1-1000 µl in 1 µl increments.
Dispensing speed: 25 s, 96-well plate, 5 µl/well
Accuracy: ±3 µl average for volumes 5 µl and above
Precision: 5-19 µl < 5%, 20-1000 µl < 2%
General features
Sample format: 1 - 384-well plates
Measurement speed: 15 s, 96-well plate
Wavelength selection: Filters
Light source: Quartz-halogen lamp
Detector: Photomultiplier tube
Incubator: From ambient +3°C to 45°C, at ambient 25°C
Shaking: Orbital shaker
User interface: Requires, but does not include a personal computer
Computer interface: Serial RS-232C port
Series Modals
Thermo Scientific - Fluorometry Specifications
[+] View Model Specifications| Depth | 16.5 in |
| Detector | Photomultiplier tube |
| Height | 13.4 in |
| Detection Mode | Mid-level |
| Width | 16.5 in |
| Shaking Pattern | Orbital shaker |
| Filters | Excitation filters: Up to eight in the excitation filter wheel. 355 nm and 485 nm filters included as standard. Other filters available upon request. Emission filters: Up to six filters in the emission filter wheel. 460 nm and 538 nm filters included as standard. Other filters available upon request. |
| Sample Formats | 1 - 384-well plates |
| Lighting | Quartz-halogen lamp |
| Weight | 46 lbs |
| Scanning Speed | 15 s, 96-well plate |
| Wavelength Selection | Filters |
| Additional Model Specifications | Note: 3 optional dispensers add 7.7 lbs to the weight. Sensitivity: 2 fmol fluorescein/well in a black 96-well plate Dynamic range: >6 decades Dispensing Number of dispensers: Up to 3 Dispensing volume: 1-1000 µl in 1 µl increments. Dispensing speed: 25 s, 96-well plate, 5 µl/well Accuracy: ±3 µl average for volumes 5 µl and above Precision: 5-19 µl < 5%, 20-1000 µl < 2% General features User interface: Requires, but does not include a personal computer Computer interface: Serial RS-232C port |
Thermo Scientific - Luminometry Specifications
[+] View Model Specifications| Depth | 16.5 in |
| Detector | Photomultiplier tube |
| Height | 13.4 in |
| Detection Mode | Mid-level |
| Width | 16.5 in |
| Shaking Pattern | Orbital shaker |
| Sample Formats | 1 - 384-well plates |
| Lighting | Quartz-halogen lamp |
| Weight | 46 lbs |
| Scanning Speed | 15 s, 96-well plate |
| Wavelength Selection | Filters |
| Additional Model Specifications | Note: 3 optional dispensers add 7.7 lbs to the weight. Sensitivity: 40 amol ATP/well using flash reaction, white 384-well plate Dynamic range: > 9 decades over whole gain setting area Dispensing Number of dispensers: Up to 3 Dispensing volume: 1-1000 µl in 1 µl increments. Dispensing speed: 25 s, 96-well plate, 5 µl/well Accuracy: ±3 µl average for volumes 5 µl and above Precision: 5-19 µl < 5%, 20-1000 µl < 2% General features User interface: Requires, but does not include a personal computer Computer interface: Serial RS-232C port |