Do you need a faster, more reliable solution for DNA fragmentation and library construction? The NEBNext® Ultra™ II FS DNA Library Prep Kit meets the dual challenges of constructing high quality libraries from ever-decreasing input amounts, and scalability of library construction. 
 
The kit includes a new DNA fragmentation reagent, which is also combined with end repair and dA-tailing reagents, enabling these steps to be performed in the same tube, with no clean-ups or sample loss. 
 
You’ll be thrilled to pieces with the result – a reliable, flexible, high-quality library prep that is fast and scalable. 
 
Perform fragmentation, end repair and dA-tailing with a single enzyme mix  
Experience reliable fragmentation with a single protocol, regardless of DNA input amount or GC content  
Prepare high quality libraries from a wide range of input amounts: 100 pg–500 ng  
Generate high yields with increased reaction efficiencies and minimized sample loss  
Use with DNA in standard buffers (TE, Tris-HCl) and water  
Save time with a streamlined workflow: ~ 2.5 hours, with < 15 minutes hands-on time  
Vary incubation time to generate a wide range of insert sizes