Bio-Rad Laboratories, Inc. - StarBright™ Blue 700 Fluorescent Secondary Antibodies
StarBright Blue 700 Secondary Antibodies are ideal for fluorescent western blotting — either for the detection of a...
StarBright Blue 700 Fluorescent Secondary Antibodies can be used with traditional fluorophores like the DyLight 800 Fluorophore for multiplexing. In addition, StarBright Antibodies can be used with Bio-Rad's stain-free technology and/or the hFAB™ Rhodamine Anti-Housekeeping Primary Antibodies for protein normalization. These antibodies are optimized for use with the ChemiDoc™ MP Imaging System, permitting detection of up to three proteins in a single blot.
Schematic representation of a StarBright Blue 700 Label conjugated to an antibody. The light-absorbing monomer is excited at 470 nm and, via an efficient intramolecular transfer process, the energy is transmitted to a light-emitting monomer, which emits at 700 nm. An antibody is conjugated to linker monomers.
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Features of StarBright™ Blue 700 Fluorescent Secondary Antibodies
Features and Benefits
Easy multiplexing — StarBright Secondary Antibodies can be used with IRDye 800 or other common fluorophores to detect multiple proteins on the same blot * Short exposure time — the exceptional brightness of the StarBright Fluorophore leads to shorter exposure times compared to traditional fluorescent dyes: seconds vs. minutes * High signal-to-noise ratio — StarBright Secondary Antibodies emit in the red/far red, where there is minimal background (Exmax/Emmax = 470 nm/700 nm) * Low nonspecific binding — StarBright Fluorophore is conjugated to highly cross-adsorbed antibodies
Applications and Uses StarBright Blue 700 Secondary Antibodies can be used for multiplex fluorescent western blotting with the right combination of fluorophores as described in the table below. These fluorescent secondary antibodies can also be used to detect post-translational modifications like phosphorylation of a target protein.
General Specifications
There are no General Specifications available.