MTI-GlobalStem - PluriQ™ G9™ Cloning Medium

Manufactured by  MTI-GlobalStem
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Clonal Expansion of Single Pluripotent Stem Cells

Pluripotent stem cells are known for the need to grow as colonies in order to prevent differentiation. This adds complexity to the isolation/expansion of clonal populations following research techniques such as gene editing. Have you ever wished you could isolate and expand clonal lines of pluripotent stem cells via limiting dilution starting from a single cell? Now you can. 
 
PluriQ™ G9™ Cloning Medium (100 mL) supports single cell plating and clonal expansion of human iPS or ES cells while maintaining pluripotency. From cells in culture, dissociate to single cells, set up a serial dilution to get to 10 cells/mL in PluriQ™ G9™ Cloning Medium and plate 100 µl such that the majority of cell containing wells will have a single cell. Feed daily with PluriQ™ G9™ Cloning Medium according to the protocol and in 2 weeks the cells will have expanded such that the clonal line can be split to create duplicate plates for freezing and screening or to move into a larger plate for further expansion. By conditioning our PluriQ™ G9™ Maintenance Medium on proprietary human stromal cells, the complete PluriQ™ G9™ Cloning Medium supports single cell survival and expansion without the need for additional supplements. Clonal human ES and iPS cells can subsequently be expanded in our regular PluriQ™ G9™ Maintenance Medium on G9™ VTN for further characterization and experimentation in continuous feeder-free, xeno-free conditions. PluriQ™ G9™ Cloning Medium was optimized for use with human Pluripotent Stem Cell Lines (PSCs) grown in PluriQ™ G9™ Maintenance Medium, but it is also compatible with starting cultures grown in other media such as mTeSR1™ and Essential 8™.
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Features of PluriQ™ G9™ Cloning Medium
  • Supports pluripotent stem cell expansion from a single cell * Maintains pluirpotency * Ready to use, just thaw. * Xeno-free, feeder-free. * Compatible coming from PluriQ™ G9™ Medium and other popular commercial media and plating on G9™ VTN (vitronectin) as well as other common matrices.
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