BioTek - Synergy 2
Synergy 2 Multi-Mode Microplate Reader
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Documents & Manuals
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Features of Synergy 2
- Measurement Techniques: Fluorescense Intensity, Time-Resolved Fluorescence, Fluorescence Polarization, Glow and Flash Luminescence, UV-Vis Absorbance, FRET, TR-FRET, BRET, Area Scanning, Spectral Scanning
- Unique Optical System: a narrow band monochromator, optimized filter/dichroic mirror pairing, and three broad spectrum light sources achieve superior performance in all read modes
- Compatible with Take3 Multi-Volume Plate: sixteen 2 µL microspots for low volume DNA/RNA 260 nm measurements. Saves precious samples, very fast and simple process.
- High Sensitivity: dedicated optical elements optimized for each specific modality result in the lowest limits of detection available in one compact instrument
- Modular Design: purchase only what you need and easily upgrade later as experimental requirements expand
- High Throughput: optimized for screening assays including 1536-well read mode
General Specifications
There are no General Specifications available.
Additional Specifications
General
Detection method: Fluorescence, Time Resolved Fluorescence, Fluorescence Polarization, Alpha Screen/ AlphaLisa, Luminescence, UV-Visible Absorbance
Read method: End-point, kinetic, spectral scanning, well-area scanning
Microplate types:
6- to 1536-well plates (luminescence 1-384)
PCR plates
Compatible with Take3 Multi-Volume Plate with 2 µL microspots
Temperature control: 4°C above ambient to 65°C
Shaking: Yes
Software:
Gen5™ Included
Control through USB or serial port
Automation: Yes
Absorbance
Light source: SQ Xenon Flash
Wavelength selection: Monochromator
Wavelength range: 200 - 999 nm, 1 nm increment
Bandpass: 2.4 nm
Dynamic range: 0 - 4.0 OD
Resolution: 0.0001 OD
Pathlength correction: Yes
Monochromator wavelength accuracy: +/- 2 nm
Monochromator wavelength repeatability: +/- 0.2 nm
OD accuracy:
6 decades
Bandpass: Filter dependent
Detection system: PMT
Reading speed:
384: 22 seconds
1536: 42 seconds
Luminescence
Sensitivity: 10 amol ATP typical (flash)
Wavelength range: 300 - 700 nm
Dynamic range: > 6 decades
Detection system: Low noise PMT
Fluorescence Polarization
Light source: Tungsten Halogen High Energy DPR Xenon Flash (optional)
Sensitivity: 3 mP at 1 nM fluorescein typical
Wavelength selection: Deep blocking filters/mirrors
Wavelength range: 400 - 700 nm
Time-Resolved Fluorescence
Light source: High Energy DPR Xenon Flash
Sensitivity: Europium 60 fM typical (12 amol/well 96-well plate; 6 amol/well 384-well plate)
Wavelength selection: Deep blocking filters/mirrors
Wavelength range: 200 - 700 nM (900 nm option)
Reagent Dispensers
Number: 2 syringe pumps
Dispense volume: 5 - 1000 µL in 1 µL increment
Dead volume: 1.1 mL, 100 µL with backflush
Series Modals
BioTek - Absorbance
[+] View Model SpecificationsBandwidth | 2.4 nm |
Depth | 17.5 in |
Detector | PMT |
Height | 14.5 in |
Detection Mode | Multi-label |
Width | 17 in |
Detection Method | Fluorescence, Time Resolved Fluorescence, Fluorescence Polarization, Alpha Screen/ AlphaLisa, Luminescence, UV-Visible Absorbance |
Sample Formats | 6- to 1536-well plates (luminescence 1-384), PCR plates. Compatible with Take3 Multi-Volume Plate with 2 µL microspots. |
Lighting | SQ Xenon Flash |
Weight | 60 lbs |
Read Method | End-point, kinetic, spectral scanning, well-area scanning |
Wavelength Range | 200 to 999 nm |
Scanning Speed | 96: 11 seconds, 384: 22 seconds, 1536: 42 seconds |
Wavelength Selection | Monochromator |
Additional Model Specifications | General Shaking: Yes Software: Gen5™ Included Control through USB or serial port Automation: Yes Absorbance Performance Dynamic range: 0 - 4.0 OD Resolution: 0.0001 OD Pathlength correction: Yes Monochromator wavelength accuracy: +/- 2 nm Monochromator wavelength repeatability: +/- 0.2 nm OD accuracy: < 1% at 2 OD typical < 3% at 3.0 OD typical OD linearity: < 1% from 0 to 3.0 OD typical OD repeatability: < 0.5% at 2.0 OD typical Stray light: 0.03% at 230 nm typical Reagent Dispensers Number: 2 syringe pumps Dispense volume: 5 - 1000 µL in 1 µL increment Dead volume: 1.1 mL, 100 µL with backflush |
BioTek - Fluorescence Polarization
[+] View Model SpecificationsDepth | 17.5 in |
Height | 14.5 in |
Detection Mode | Multi-label |
Width | 17 in |
Detection Method | Fluorescence, Time Resolved Fluorescence, Fluorescence Polarization, Alpha Screen/ AlphaLisa, Luminescence, UV-Visible Absorbance |
Sample Formats | 6- to 1536-well plates (luminescence 1-384), PCR plates. Compatible with Take3 Multi-Volume Plate with 2 µL microspots. |
Lighting | Tungsten Halogen High Energy DPR Xenon Flash (optional) |
Weight | 60 lbs |
Read Method | End-point, kinetic, spectral scanning, well-area scanning |
Wavelength Range | 400 to 700 nm |
Wavelength Selection | Deep blocking filters/mirrors |
Additional Model Specifications | Shaking: Yes Software: Gen5™ Included Control through USB or serial port Automation: Yes Sensitivity: 3 mP at 1 nM fluorescein typical Reagent Dispensers Number: 2 syringe pumps Dispense volume: 5 - 1000 µL in 1 µL increment Dead volume: 1.1 mL, 100 µL with backflush |
BioTek - Lumienscence
[+] View Model SpecificationsDepth | 17.5 in |
Detector | Low noise PMT |
Height | 14.5 in |
Detection Mode | Multi-label |
Width | 17 in |
Detection Method | Fluorescence, Time Resolved Fluorescence, Fluorescence Polarization, Alpha Screen/ AlphaLisa, Luminescence, UV-Visible Absorbance |
Sample Formats | 6- to 1536-well plates (luminescence 1-384), PCR plates. Compatible with Take3 Multi-Volume Plate with 2 µL microspots. |
Weight | 60 lbs |
Read Method | End-point, kinetic, spectral scanning, well-area scanning |
Wavelength Range | 300 to 700 nm |
Additional Model Specifications | General Shaking: Yes Software: Gen5™ Included Control through USB or serial port Automation: Yes Luminescence Performance Sensitivity: 10 amol ATP typical (flash) Dynamic range: > 6 decades Reagent Dispensers Number: 2 syringe pumps Dispense volume: 5 - 1000 µL in 1 µL increment Dead volume: 1.1 mL, 100 µL with backflush |
BioTek - Time-Resolved Fluorescence
[+] View Model SpecificationsDepth | 17.5 in |
Height | 14.5 in |
Detection Mode | Multi-label |
Width | 17 in |
Detection Method | Fluorescence, Time Resolved Fluorescence, Fluorescence Polarization, Alpha Screen/ AlphaLisa, Luminescence, UV-Visible Absorbance |
Sample Formats | 6- to 1536-well plates (luminescence 1-384), PCR plates. Compatible with Take3 Multi-Volume Plate with 2 µL microspots. |
Lighting | High Energy DPR Xenon Flash |
Weight | 60 lbs |
Read Method | End-point, kinetic, spectral scanning, well-area scanning |
Wavelength Range | 200 to 700 nm |
Wavelength Selection | Deep blocking filters/mirrors |
Additional Model Specifications | Shaking: Yes Software: Gen5™ Included Control through USB or serial port Automation: Yes Sensitivity: Europium 60 fM typical (12 amol/well 96-well plate; 6 amol/well 384-well plate) Reagent Dispensers Number: 2 syringe pumps Dispense volume: 5 - 1000 µL in 1 µL increment Dead volume: 1.1 mL, 100 µL with backflush |
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BioTek's Synergy 2