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Thermo Scientific - Pierce Firefly Luciferase Glow Assay Kit
Manufactured by Thermo Scientific

Thermo Scientific - Pierce Firefly Luciferase Glow Assay Kit
DESCRIPTION
A highly sensitive luciferase reporter assay system with stable signal output.

The Thermo Scientific Pierce Firefly Luciferase Glow Assay Kit provides a bright and stable bioluminescence signal (half-life greater than 2 hours) in the presence of firefly luciferase reporters for protein expression assays.      This Pierce Luciferase Glow Assay Kit contains reagents for measuring the activity of firefly luciferase in cell lysates. In the presence of firefly luciferase, the glow reagent provides stable bioluminescent signal and is highly suited for luminometers without injectors or for batch processing of samples. The light output generated by the luciferase reaction can be correlated with the amount of luciferase protein produced which in turn is proportional to the promoter activity driving the luciferase expression. The glow assay reagents were optimized for best results with Thermo Scientific Firefly Luc Plasmids; however, this assay kit may be used to detect the activity of other firefly or ATP-dependent luciferases that use D-luciferin as a substrate.

FEATURES

Sensitive – highly sensitive detection of firefly luciferase activity; for best results (maximum signal) use with Pierce Firefly Luciferase Signal Enhancer   Stable – increased signal stability compared to flash assays   Simple – does not require a luminometer with injectors   Compatible – assay reagents compatible with other firefly beetle luciferases   Automation-friendly – amenable to high throughput assays   Convenient – contains a universal cell lysis buffer and optimized glow assay reagent   Safe – allows one to perform non-radioactive assays

SPECIFICATIONS
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Thermo Scientific - Pierce Firefly Luciferase Glow Assay Kit
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