The Thermo Scientific Pierce Firefly Luciferase Glow Assay Kit provides a bright and stable bioluminescence signal (half-life greater than 2 hours) in the presence of firefly luciferase reporters for protein expression assays. This Pierce Luciferase Glow Assay Kit contains reagents for measuring the activity of firefly luciferase in cell lysates. In the presence of firefly luciferase, the glow reagent provides stable bioluminescent signal and is highly suited for luminometers without injectors or for batch processing of samples. The light output generated by the luciferase reaction can be correlated with the amount of luciferase protein produced which in turn is proportional to the promoter activity driving the luciferase expression. The glow assay reagents were optimized for best results with Thermo Scientific Firefly Luc Plasmids; however, this assay kit may be used to detect the activity of other firefly or ATP-dependent luciferases that use D-luciferin as a substrate.
Sensitive – highly sensitive detection of firefly luciferase activity; for best results (maximum signal) use with Pierce Firefly Luciferase Signal Enhancer Stable – increased signal stability compared to flash assays Simple – does not require a luminometer with injectors Compatible – assay reagents compatible with other firefly beetle luciferases Automation-friendly – amenable to high throughput assays Convenient – contains a universal cell lysis buffer and optimized glow assay reagent Safe – allows one to perform non-radioactive assays