Abcam - SimpleStep ELISA Kits

Manufactured by  Abcam
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SimpleStepTM ELISA is a single wash colorimetric sandwich ELISA assay providing improved and differentiated performance characteristics.

SimpleStepTM ELISA is a single wash colorimetric sandwich ELISA assay providing improved and differentiated performance characteristics while still retaining the familiar process and standard data outputs of a traditional ELISA kit.       
       
The SimpleStepTM ELISA kit employs a labeled capture and detection antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/protein/detection antibody) is in turn immobilized in the well via immunoaffinity of an anti-tag antibody coated in the microplate well.
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Features of SimpleStep ELISA Kits

Simplicity - Single wash step assay greatly simplifies and abbreviates the overall assay method        Ease - Platform requires no specific training or new instrumentation        Accuracy - Reduced sample handling steps yield greater reproducibility compared with techniques requiring multiple wash and incubation steps        Binding kinetic advantages - In addition to time saving, antibodies interacting with sample analyte in solution reflect a more efficient binding process driving better sensitivity and specificity than with immobilized immunocapture

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