Agilent Technologies - 3100 OFFGEL Fractionator

Manufactured by  Agilent Technologies
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Protein fractionation, in-solution recovery

Prefractionation of proteins and peptides prior to LC/MS analysis can dramatically increase the number of proteins ultimately identified. The Agilent 3100 OFFGEL Fractionator uses a novel isoelectric focusing technique to achieve excellent pI-based fractionation. The resulting fractions are in solution, making recovery for LC/MS analysis much easier than with old-fashioned gels. Results are highly reproducible, and the Agilent 3100 OFFGEL Fractionator is simple and easy to use.
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Features of 3100 OFFGEL Fractionator
  • pI-based OFFGEL fractionation with 0.1 – 0.6 pH resolution depending on consumable kit used
  • In-solution fraction recovery for easy transfer to LC/MS
  • Two power supplies allow simultaneous fractionation of two sample sets with broad differences in concentration
  • Up to 16 samples fractionated at the same time
  • µg to mg load capacity
  • Possible to run conventional in-gel IEF as well as OFFGEL fractionation
  • Preconfigured methods for OFFGEL and in-gel modes
  • Online current control for each individual sample
  • Simple, reproducible, and easy to use
General Specifications
Depth 16.8 in
Height6.2 in
Width 14 in
Power Requirements 100-240 VAC, 50/60 Hz, 140 VA
Weight31 lbs
Additional Specifications

Temperature Range: -40 to 70°C Ambient non-operating
Humidity: < 80% at 40° C (104 °F) Non-condensing
Operating altitude: Up to 2000 m (6500 ft)
Non-operating altitude: Up to 4600 m (14950 ft)
Safety standards IEC, CSA, UL: Installation Category II, Pollution Degree 2
Platform temperature:
Range: 10 - 35 °C
Max. 10 °C below ambient
Non condensing
Power supplies:
2 independent high voltage power supplies
Voltage range: 0.5–10kV
Current range: 0-150 uA/ strip
Power: 0-1 W/strip
Modes:
Constant voltage
Constant current
Contant power
Timetable programable
(Individual current control for each sample)
Fractionation capacity: 2 x 8 samples (12 or 24 fractions) in OFFGEL mode and IPG-IEF (in gel) on 2 individual trays
Resolution: 0.1 - 0.6 pH units between neighboring fractions, depending on kit used
Loading capacity: 50 µg – 5 mg of total protein or peptide amount per sample
Content: Each kit contains all chemical and consumables for 12 OFFGEL fractionations

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