Molecular Devices - SpectraMax i3x Multi-Mode Microplate Platform
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Multi-Mode Microplate Reader Platform. Sensitive absorbance, luminescence, and fluorescence microplate detection platform that evolves over time.
The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes.
With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user-configurable detection modules, the reader provides you with the ability to explore cellular pathways and protein expression on one microplate reader.
User-configurable applications
User-configurable detection modules expand the reader’s detection capabilities to include AlphaScreen, Time-Resolved Fluorescence, HTRF, fast kinetics with injectors, western blot detection, and more.
No-stain cell analysis
The StainFree™ Cell Detection Algorithm for brightfield cell segmentation enables cell counting and confluency measurement without destructive stains, simplifying the cell counting workflow.
Multi-channel functionality
Two fluorescence detection channels on the MiniMax cytometer enable the analysis of cell viability or cell toxicity assays, including ratiometric assays like live-dead and transfection efficiency.
Cellular pathway analysis
Cell confluence and cell viability imaging, western blot analysis, and quantitation of nucleic acids and protein, can be captured on one reader.
Other features and benefits include:
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Features of SpectraMax i3x Multi-Mode Microplate Platform
User-Upgradeability Expand the 3-mode base system with additional application cartridges for FP, HTRF®, AlphaScreen® assays, and cellular imaging
SpectraMax® MiniMax™ Imaging Cytometer Simplify complex imaging workflow by visualizing cell morphology changes on top of well-based intensity readings
Spectral Fusion™ Illumination Delivers high-powered full spectrum light source for increased fluorescence performance across the entire excitation range Expanded Dynamic Range Sophisticated engineering delivers optimal sensitivity and maximizes signal range
SoftMax® Pro Microplate Data Acquisition & Analysis Software Industry-leading SoftMax Pro Software streamlines complex data analysis with over 140 built-in protocols, effectively eliminating the need for additional software. Additional tools for regulatory compliance are included in SoftMax Pro GxP Software
General Specifications
| Depth | 23.38 in |
| Detector | PMT and/or photodiode |
| Shaking | 1 |
| Height | 12.63 in |
| Detection Mode | Multi-label |
| Width | 15.38 in |
| Power Requirements | 100-240 Vac, 4 A, 50/60 Hz |
| Shaking Pattern | linear, orbital |
| Peak Power | 200 W |
| Lighting | Spectral Fusion Illumination (Xenon flash lamp + high-powered LEDs or laser diode in detection cartridges) |
| Weight | 36 lbs |
| Wavelength Range | 230 to 1000 nm |
| Microplate Size | 384-well |
| Software | External |
| Temperature Control | 1 |
Additional Specifications
General Photometric Performance
Plate formats:
6 to 1536 wells§
Reading capabilities:
Microplates, cuvettes (via adapter)
Shaking:
Linear and orbital
Injectors:
Option available
Temperature control:
4°C above ambient to 45°C
Temperature uniformity:
±0.75°C
Temperature accuracy:
±1°C at 37°C set point
Environmental control:
Gas quick connect
Endpoint reading:
All modes
Kinetic reading:
All modes
Spectral scanning:
Abs, Fl, Lum
Well scanning:
Over 20 by 20 in all modes
Wavelength selection:
1.0 nm increments
Absorbance Photometric Performance
Wavelength range:
230-1000 nm
Wavelength bandwidth:
4.0 nm
Wavelength accuracy:
± 2.0 nm
Wavelength repeatability:
± 1.0 nm
Photometric range:
0-4.0 OD
Photometric resolution:
0.001 OD
Photometric accuracy:
6 logs
Luminescence Performance
Wavelength range:
250-850 nm
Wavelength selection:
Choice of simultaneous detection of all wavelenths or selection in 1.0 nm increments
Dynamic range:
> 6 decades
Cross-talk:
< 0.3% in white 96- and 384-well microplates
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SpectraMax i3 Multi-Mode Microplate Reader Platform