Applied Biosystems - QuantStudio™ 12K Flex

Manufactured by  Applied Biosystems
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The QuantStudio™ 12K Flex system is a highly flexible, comprehensive real-time PCR platform that leverages the ...

The QuantStudio™ 12K Flex system is a highly flexible, comprehensive real-time PCR platform that leverages the best of Applied Biosystems real-time technology in a single instrument. The QuantStudio™ 12K Flex Real-Time PCR System includes easy-to-change blocks that can accommodate OpenArray® plates, TaqMan® array cards, 384-well, 96-well, and FAST 96-well plates allowing you to select the format that is right for your project whether it’s now or in the future, for maximum flexibility and consistency in your research.

The QuantStudio™ 12K Flex System’s intuitive software, easy touch screen setup, automation integration, effortless block exchange, and easy-to- use OpenArray® components are designed to let you get started right away and reduce hands on time. Plan for the future and save time, money, and space in your lab with the only all-in-one qPCR instrument enabling a broad range of applications with maximum throughput.

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Features of QuantStudio™ 12K Flex

Flexibility & Scalability

Includes five interchangeable blocks:

The QuantStudio™ 12K Flex blocks accomodate 96-well, FAST 96-well, 384-well, TaqMan® array cards, and OpenArray® plates, for effortless scaling from 1 to 12,000 data points in a single run. Increase throughput as you dive deeper into your research, using just one instrument. As you begin your project, start with 96-well format experiments and then scale up to a higher throughput to get the statistically significant answers you need. Additionally, you can easily validate and screen your targets of interest with a selected panel of genes using novel formats such as the TaqMan® array cards and OpenArray® plates.

Compatible with a full range of TaqMan® assays:

The QuantStudio™ 12K Flex System is optimized to enable clear, clean data for all TaqMan® assays that might be used across a broad range of applications, including gene expression, microRNA and small noncoding RNAs, SNP genotyping, protein analysis, protein thermal shift, high resolution melt, copy number variation, and pathogen detection.

Provides a seamless switch from qPCR to digital PCR:

Increase the precision and sensitivity of your nucleic acid experiment by switching from qPCR to digital PCR mode using QuantStudio™ Digital PCR kits and DigitalSuite™ Software with the QuantStudio™ OpenArray® block.

Delivers peace of mind:

Leave early on a Friday and monitor runs from home, knowing that the system can safely store over 100 runs.

Maximum Throughput

Enhanced OptiFlex® System:

Building upon Applied Biosystems’ proprietary technology, the Enhanced OptiFlex® System provides enhanced fluorescence detection for the accurate and sensitive data analysis necessary to collect 12,000 data points in a single run.

Unprecedented coverage and sample throughput:

The OpenArray® format accelerates genomic validation and screening, driving over 150,000 data points per work day, enabling most projects to be completed in days instead of weeks.

Simple OpenArray® workflow:

QuantStudio™ Open Array® plates arrive in a case to facilitate ease of handling and ensure optimal data quality upon loading of samples using the Accufill™ System. Easily load four arrays into the QuantStudio™ 12K Flex instrument to achieve over 12,000 data points. Integrated sample tracking software lets you easily track your samples throughout the course of your project.

Integrated analysis and quality systems:

New intuitive touch-screen interface and integrated calendar feature allows for scheduling time of use on the instrument. Additional analysis software such as DigitalSuite™ v1.0, ExpressionSuite v1.0, and Genotyper v1.1 is available to help you analyze hundreds of targets and samples and achieve the statistical power you need.

QuantStudio™ 12K Flex Software

  • Intuitive interface and innovative design for novice and experienced users
  • Convenient walk-away operation
  • Improved data analysis across block types
  • Fully compatible with high-throughput environments

Expandable Software Architecture

Optional add-ons are available to upgrade the existing software:

  • 21 CFR Part 11 compliance: This optional SAE module assists with Security, Auditing, and Electronic signature of records for full data traceability.
  • High Resolution Melt (HRM): Using the built-in MeltDoctor™ protocols and calibrations, you can spend less time optimizing the run and more time customizing your results. Define the number of variants you want to see by quickly changing analysis settings.
  • DigitalSuite™ Software v1.0: For OpenArray® block users needing sensitivity for rare targets and precision to measure small differences in target abundance.
  • ExpressionSuite Software v1.0: Life Technologies’ latest stand-alone gene expression software with enhanced analysis tools that can analyze over 200 384-well plates or 10 OpenArray® plates.
General Specifications
Depth 67.2 cm
Height73.8 cm
Width 50.5 cm
Power Requirements 100–240 V, ~50/60 Hz
Weight152 lbs
Additional Specifications

Supported Blocks:  
96-well standard (10100 µL reactions) 
96-well Fast (1030 µL reactions) 
384-well (520 µL reactions) 
TaqMan® Array Micro Fluidic Cards (~1 µL reactions) 
QuantStudio™ 12K Flex OpenArray™ Plates (~33 nL reactions) 
Excitation Source:  
Enhanced OptiFlex™ System - Whilte LED 
Instrument Control:  
Instrument touch-screen OR networked computer OR attached computer 
Block Change Design:  
Block change from front of instrument in less than 1 min; no secondary tools required 
Run Time:  
30 minutes expected (Fast 96-well) 
35 minutes (384-well) 
2 hrs (OpenArray™) (gene expression run) 
4 hrs (OpenArray™) (genotyping run) 
Dynamic Range:  
Up to 9 logs of linear dynamic range 
Up to 7 logs of linear dynamic range (OpenArray™) 
Resolution:  
Detect as little as 1.5-fold changes in target quantities in singleplex reaction 
21 CFR p11 Enablement:  
Optional software module 
Automation:  
Includes plate-loading arm w/ barcode (sales, service, and support provided by Life Technologies) 
Remote Monitoring:  
Ability to monitor up to 10 networked instruments simultaneously 
Remote Notification:  
Emails user when run is started, stopped, or aborted 
Run data attached to email 
Data Export Format:  
Fully configurable by user

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