Applied Biosystems - ViiA™ 7

Manufactured by  Applied Biosystems
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The ViiA™ 7 Real-Time PCR System puts high-productivity qPCR at your fingertips. With a streamlined workflow,...

The ViiA™ 7 Real-Time PCR System puts high-productivity qPCR at your fingertips. With a streamlined workflow, one-button protocol feature, and intuitive software, the ViiA™ 7 Real-Time PCR System is faster to set up, easier to run, and more convenient to automate than any other qPCR platform. Using the ViiA™ 7 System with TaqMan® Array Micro Fluidic Cards provides an integrated workflow and faster results. And with the OptiFlex™ optical system, you’ll have greater dye flexibility for multiplexing than ever before. The end result is high-accuracy data with minimal distraction, which means you can stay focused on what matters most—your research.
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Features of ViiA™ 7

Productivity  Ideal for performing medium- to high-throughput real-time PCR, the ViiA™ 7 System enhances your lab’s productivity. 

  • Quick block changes: Front access makes it easy to change thermal cycling block formats without having to move attached peripherals such as robots or computers. 
  • Easy touch-screen interface: Instrument touch screen provides one-touch protocols for fast and easy assay setup for a broad range of applications. 
  • Automation compatibility: Integrated with Applied Biosystems® Twister® II Robot, the ViiA™ 7 System allows you to maximize productivity for automated environments. 
  • Peace of mind: Data recovery capability for 100+ runs. 

Integration  Fully Compatible with TaqMan® Array Micro Fluidic Cards, Applied Biosystems® TaqMan® Assays are the gold standard in qPCR analysis, providing high sensitivity, high specificity, and a broad dynamic range. 

  • TaqMan® Array-ready: Hundreds of customizable and predefined TaqMan® Array Micro Fluidic Cards are available, containing preloaded TaqMan® Gene Expression or MicroRNA Assays in an easy-to-use format. You can analyze 1–8 samples in parallel using your choice of 12–384 TaqMan® Assays. 
  • 384-well throughput without robotics: With TaqMan® Array Micro Fluidic Cards, there’s no need for liquid-handling robotics or complex pipetting to load; simply add your sample and master mix and run on the ViiA™ 7 System. 
  • Compatible with the full range of TaqMan® Assays: The ViiA™ 7 System is optimized to enable clear, clean data for all TaqMan® Assays, including MicroRNA Assays, Protein Assays, Long Non-coding RNA Assays, and Pri-miRNA Assays. 

Performance  The ViiA™ 7 System features the new OptiFlex™ System, which provides enhanced fluorescence detection enabling accurate and sensitive data analysis. 

  • Better accuracy: High-resolution detection system for more accurate reads with TaqMan® Array Micro Fluidic Cards, 384-well plates, and 96-well plates. 
  • Maximum multiplexing: Six decoupled excitation and emission filter channels for the greatest number of dye combinations and maximum multiplexing capabilities. 
  • Flexible data collection: Multiple ramp method detection formats provide more flexibility for collecting data during a ramp stage. 
  • Precise quantification: Detect as small as 1.5–fold changes in target quantities in singleplex reactions. 

ViiA™ 7 Software  ViiA™ 7 software drives value in a number of ways, offering: 

  • Intuitive interface and innovative design 
  • Convenient walk-away operation 
  • Improved data analysis 
  • Fully compatible with high-throughput environments 

Expandable Software Architecture  Optional add-ons to upgrade features to existing software 

  • 21 CFR Part 11 compliance: The optional SAE module assists with Security, Auditing and Electronic signature of records for full data traceability. 
  • High Resolution Melt (HRM): Using the built-in MeltDoctor™ protocols and calibrations, you can spend less time optimizing the run and more time customizing your results. Define the number of variants you want to see by quickly changing analysis settings.
General Specifications
Depth 25 in
Height25.4 in
Real-Time PCR 1
Width 21 in
Power Requirements 100–240 V, ~50/60 Hz
Weight134 lbs
Additional Specifications

Temperature Uniformity:  
±0.5°C, within 30 sec of reaching temperature set point 
Demonstrated Sensitivity:  
Down to 1 copy 
Block Change Design:  
Block change from front of instrument in less than 1 min; no secondary tools required 
Supported Applications:  
Standard curve, Comparative Ct, Genotyping, Presence/Absence, HRM, Melt curve 
Run Time:  
~35 min for 96/384-well blocks, using Applied Biosystems® Fast Master Mix 
Supported Blocks and Volume:  
96-well standard (10–100 ΜL reactions) 
96-well Fast (10–30 ΜL reactions) 
384-well (5–20 ΜL reactions) 
TaqMan® Array Micro Fluidic Cards (~1 ΜL reactions) 
Excitation/Detection Wavelengths:  
6 excitation (450–670 nm) 
6 emission (500–720 nm) 
Dynamic Range:  
Up to 9 logs of linear dynamic range 
Resolution:  
Detect as little as 1.5-fold changes in target quantities in singleplex reaction 
21 CFR p11 Enablement:  
Security, auditing, and eSigniture features for instrument data 
Automation:  
Includes plate-loading arm w/ barcode (Sales, service, and support provided by Life Technologies) 
Remote Monitoring:  
Ability to monitor 15 instruments at a time; optional Smart Monitoring Service for remote instrument diagnostic monitoring by Life Technologies 
Remote Notification:  
Emails user when run is started, stopped, or aborted 
Data Export Format:  
Fully configurable by user

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