Thermo Scientific - EASY-nLC 1000
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The Thermo Scientific EASY-nLC 1000 provides effortless split-free, nano-flow UHPLC performance up to 1000 bar with...
Identify more peptides and increase productivity with higher pressure without compromising reliability and robustness.
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Features of EASY-nLC 1000
Advanced flow control
- Intelligent Flow Control (IFC) during loading and equilibration provides robust performance and protects against blocked columns
- Advanced Flow Control (AFC) during gradients provides outstanding reproducibility, sample to sample and over time
Built-in PC & Maintenance
- Automated, built-in maintenance steps
- Remote access via internet for system interrogation
- Event log for troubleshooting
- Automated leak test indicates actual position of leak
- System back pressure test quickly detects flow-line blockage
Integrated design
- Structured layout for easy component exchange
- Pulsation-free pumps minimize need for replacement of seals, valves and fittings
- Optimized flow paths are utilized by software
Stable, pulsation-free gradients from 100nL/min.
- Factory-configured flow paths minimize swept volume
- Maximum chromatographic accuracy with: split-free gradient mixing, step-controlled nano-flow syringe pumps, and flow sensor control of mobile phase delivery
Easily switch between one or two column configuration
General Specifications
| Depth | 15 in |
| Height | 18 in |
| Width | 14 in |
| Power Requirements | 100 - 240 V, 50 - 60 Hz |
| Peak Power | 250 VA |
| Weight | 37 kg |
Additional Specifications
Injection Loops: 20µL (5µL or 50µL optional)
Pick-up Volume Range:
0.1-18µL (20µL loop) in 0.01µL steps Pick-up
Reproducibility (Injection RSD)
0.2% at 5µL; 3.0% at 100nL
Pick-up Linearity:
BSA: 0.9985 at 0.5-10µL injection volume
Caffeine: 0.9995 at 0.3-1.6µL injection volume
Flow Range: Sample pick-up: 0- 40µL/min
Injection Pump: Single-acting syringe pump (sapphire piston), 140µL volume
Refill Speed: 40µL/min
Injection Loop Bypass Volume: 408nL
Carryover:
<0.05%. Two extra solvents (in addition to water/solvent A) are
available for repeated custom wash cycles for thorough cleaning of injection needle
Formats:
Gradient: 20-2,000 nL/min; 100-1,000 nL/min (rec.)
Loading and re-equilibration: up to 25µL/min. (back-pressure-limited)
Gradient Swept Volume: 110nL
Gradient Pumps: Single-acting syringe pumps (sapphire piston), 140µL vol.
Valves: 4 identical 6-port, 2 position micro-valves from VICI* Valco*
Pressure Range: 0 to 1000 Bar
Retention Time Reproducibility: Typically 0.1- 0.4% RSD at recommended flow
Sensitivity (Typical Peak Widths): 3 - 5 sec FWHM using 10cm, 50µm ID column with 1.9µm beads
Mobile Phase Compatibility: Wetted Parts: Fused silica, PEEK, 316 Stainless steel
MS Drivers: HyStar* 3.2, Thermo Scientific Xcalibur 1.4, 2.0 and 2.1
Remote Support Secure, direct internet access for rapid, online diagnostics by EASY-nLC experts